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MB Sample ID: SA226658
| Local Sample ID: | FIT2-LKO_03 |
| Subject ID: | SU002389 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002389 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| FIT2-LKO_03 | SA226658 | FL027496 | Liver Knockout | Genotype |
Collection:
| Collection ID: | CO002382 |
| Collection Summary: | All mice fasted 2 hours prior to sacrifice. Mice were euthanized with isoflurane and tissues were collected. |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR002401 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP002395 |
| Sampleprep Summary: | Liver (~100mg) was homogenized in 1 mL of PBS using a Bead Mill Homogenizer (VWR). Lipids were extracted, according to the Folch method. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN003763 | AN003764 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Thermo Accucore C30 (250 x 2.1mm,3um) | Thermo Accucore C30 (250 x 2.1mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Area | Area |
Chromatography:
| Chromatography ID: | CH002783 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Accucore C30 (250 x 2.1mm,3um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003506 |
| Analysis ID: | AN003763 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry data analysis was performed using LipidSearch version 4.1 SP (Thermo Fisher Scientific). The results were exported to R-Studio where quality control was performed using pairwise correlations between replicates, a principal component analysis comparing sample groups, as well as retention time plot analysis to verify elution clustering within lipid classes. All identified lipids were included for subsequent analyses if they fulfilled the following LipidSearch-based criteria: 1) reject equal to zero, 2) main grade A OR main grade B AND APvalue<0.01 for at least three replicates, and 3) no missing values across all samples. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003507 |
| Analysis ID: | AN003764 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry data analysis was performed using LipidSearch version 4.1 SP (Thermo Fisher Scientific). The results were exported to R-Studio where quality control was performed using pairwise correlations between replicates, a principal component analysis comparing sample groups, as well as retention time plot analysis to verify elution clustering within lipid classes. All identified lipids were included for subsequent analyses if they fulfilled the following LipidSearch-based criteria: 1) reject equal to zero, 2) main grade A OR main grade B AND APvalue<0.01 for at least three replicates, and 3) no missing values across all samples. |
| Ion Mode: | NEGATIVE |
