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MB Sample ID: SA188321

Local Sample ID:MMV1900328_3
Subject ID:SU002092
Subject Type:Cultured cells
Subject Species:Plasmodium falciparum
Taxonomy ID:5833
Genotype Strain:3D7

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Subject:

Subject ID:SU002092
Subject Type:Cultured cells
Subject Species:Plasmodium falciparum
Taxonomy ID:5833
Genotype Strain:3D7

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
MMV1900328_3SA188321FL02318410X IC50Concentration
MMV1900328_3SA188321FL0231842.5 hoursTreatment time
MMV1900328_3SA188321FL023184MACS purified TrophParasite Stage

Collection:

Collection ID:CO002085
Collection Summary:Cells were pelleted and the media aspirated then a 1xPBS wash that was aspirated prior to quench and extraction.
Sample Type:Pf infected RBCs

Treatment:

Treatment ID:TR002104
Treatment Summary:Purified iRBCs were treated with 10XIC50 drug for 2.5 hours

Sample Preparation:

Sampleprep ID:SP002098
Sampleprep Summary:90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation.
Processing Method:Lysis
Processing Storage Conditions:4?
Extraction Method:See SA Cobbold et al JBC 2013
Extract Cleanup:Clarification via centrifugation followed by nitrogen drying
Extract Storage:-80?
Sample Spiking:Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer

Combined analysis:

Analysis ID AN003277 AN003278 AN003279
Analysis type MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column Waters XSelect HSS T3 C18 (100 x 2.1mm,2.5um) Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um) Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um)
MS Type ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Exactive Plus Orbitrap Thermo Exactive Plus Orbitrap Thermo Exactive Plus Orbitrap
Ion Mode NEGATIVE NEGATIVE NEGATIVE
Units peak area peak area peak area

Chromatography:

Chromatography ID:CH002419
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Waters XSelect HSS T3 C18 (100 x 2.1mm,2.5um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH002420
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH002421
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um)
Chromatography Type:Reversed phase

MS:

MS ID:MS003049
Analysis ID:AN003277
Instrument Name:Thermo Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:El-Maven for feature assignments
Ion Mode:NEGATIVE
  
MS ID:MS003050
Analysis ID:AN003278
Instrument Name:Thermo Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:El-Maven for feature assignments
Ion Mode:NEGATIVE
  
MS ID:MS003051
Analysis ID:AN003279
Instrument Name:Thermo Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:El-Maven for feature assignments
Ion Mode:NEGATIVE
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