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MB Sample ID: SA184929

Local Sample ID:WT_4_70
Subject ID:SU002046
Subject Type:Bacteria
Subject Species:Bacillus cereus
Taxonomy ID:11778
Gender:Not applicable

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Subject:

Subject ID:SU002046
Subject Type:Bacteria
Subject Species:Bacillus cereus
Taxonomy ID:11778
Gender:Not applicable

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
WT_4_70SA184929FL022741Wild-typeGenotype
WT_4_70SA184929FL022741StationaryGrowth Phase
WT_4_70SA184929FL022741DABCOMDTreatment

Collection:

Collection ID:CO002039
Collection Summary:Samples were collected in screw-cap conical centrifuge tubes for all sample types in both the mid log and stationary phases. They were centrifuged, the supernatant was discarded, they were rinsed with cold 1x PBS and transferred to glass centrifuge tubes. They were centrifuged, the supernantant was discarded and the cell pellets were frozen at - 80 C.
Sample Type:Bacterial cells
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002058
Treatment Summary:Unchallenged samples of both WT and Mut bacteria were grown in Brodo Mueller Hinton II Media (BMHII), the challenged samples (WT D and Mut D) were grown in BMHII with 33 % of the MIC value DABCOMD added to the media. All other procedures were the same between the two groups.

Sample Preparation:

Sampleprep ID:SP002052
Sampleprep Summary:Hydrophilic metabolites were obtained from the aqueous layer of the methanol/water/chloroform extraction. An acetone precipitation was used to extract the proteins. Metabolite samples were prepared by taking the supernatant obtained after acetone precipitation, speed vacuuming them down and adding 700 µL of the NMR buffer. The mixture was vortexed and transferred to capped glass NMR tube.

Analysis:

MB Sample ID:SA184929
Analysis ID:AN003204
Analysis Type:NMR
Num Factors:8
Num Metabolites:37
Units:mM

NMR:

NMR ID:NM000218
Analysis ID:AN003204
Instrument Name:Bruker Avance III 600 MHz NMR
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600 MHz
NMR Probe:5 mm triple resonance (1H, 15N, 13C) liquid-helium cooled TCI NMR CryoProbe
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