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MB Sample ID: SA184615

Local Sample ID:6H-2
Subject ID:SU002040
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Not applicable

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Subject:

Subject ID:SU002040
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Not applicable

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
6H-2SA184615FL022708cultured brown adipocytes treated with 1mM of d9-choline for 6hrsSample detail

Collection:

Collection ID:CO002033
Collection Summary:Cells were harvested at the indicated time points and washed three times with ice cold 5% mannitol.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR002052
Treatment Summary:Fully differentiated cultured brown adipocytes were treated with or without 1mM of d9-choline for 1, 6 or 24 hours.

Sample Preparation:

Sampleprep ID:SP002046
Sampleprep Summary:Lipids were extracted using a methanol–chloroform–water extraction method. The methanol used contained 25 μM each of methionine sulfone and D-camphor-10-sulphonic acid and 10 μM each of 1,2-dimyristoyl(d54)-sn-glycero-3-phosphocholine and 1,2-dihexanoyl(d22)-sn-glycero-3-phosphocholine.

Combined analysis:

Analysis ID AN003195
Analysis type MS
Chromatography type Unspecified
Chromatography system none
Column none
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode POSITIVE
Units fmol/cell

Chromatography:

Chromatography ID:CH002363
Instrument Name:none
Column Name:none
Chromatography Type:Unspecified

MS:

MS ID:MS002973
Analysis ID:AN003195
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Mass spectra were acquired at the rate of 1.5 cycles /sec over an mass range of m/z100–1,700. The reference masses of reference standards were 121.050873(Purine) and 922.009798(HP-0921) for positive ion mode, and 112.985587(TFA) and 1033.988109(HP-0921+TFA) for negative ion mode. Raw data from LC–TOFMS were processed using Master Hands ver. 2.17.0.8 software for the quantification of lipids.
Ion Mode:POSITIVE
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