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MB Sample ID: SA184611
Local Sample ID: | 24H-3 |
Subject ID: | SU002040 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Not applicable |
Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002040 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Not applicable |
Species Group: | Mammals |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
24H-3 | SA184611 | FL022707 | cultured brown adipocytes treated with 1mM of d9-choline for 24hrs | Sample detail |
Collection:
Collection ID: | CO002033 |
Collection Summary: | Cells were harvested at the indicated time points and washed three times with ice cold 5% mannitol. |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR002052 |
Treatment Summary: | Fully differentiated cultured brown adipocytes were treated with or without 1mM of d9-choline for 1, 6 or 24 hours. |
Sample Preparation:
Sampleprep ID: | SP002046 |
Sampleprep Summary: | Lipids were extracted using a methanol–chloroform–water extraction method. The methanol used contained 25 μM each of methionine sulfone and D-camphor-10-sulphonic acid and 10 μM each of 1,2-dimyristoyl(d54)-sn-glycero-3-phosphocholine and 1,2-dihexanoyl(d22)-sn-glycero-3-phosphocholine. |
Combined analysis:
Analysis ID | AN003195 |
---|---|
Analysis type | MS |
Chromatography type | Unspecified |
Chromatography system | none |
Column | none |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6530 QTOF |
Ion Mode | POSITIVE |
Units | fmol/cell |
Chromatography:
Chromatography ID: | CH002363 |
Instrument Name: | none |
Column Name: | none |
Chromatography Type: | Unspecified |
MS:
MS ID: | MS002973 |
Analysis ID: | AN003195 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Mass spectra were acquired at the rate of 1.5 cycles /sec over an mass range of m/z100–1,700. The reference masses of reference standards were 121.050873(Purine) and 922.009798(HP-0921) for positive ion mode, and 112.985587(TFA) and 1033.988109(HP-0921+TFA) for negative ion mode. Raw data from LC–TOFMS were processed using Master Hands ver. 2.17.0.8 software for the quantification of lipids. |
Ion Mode: | POSITIVE |