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MB Sample ID: SA184608
| Local Sample ID: | 24H-1 |
| Subject ID: | SU002040 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002040 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Not applicable |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 24H-1 | SA184608 | FL022707 | cultured brown adipocytes treated with 1mM of d9-choline for 24hrs | Sample detail |
Collection:
| Collection ID: | CO002033 |
| Collection Summary: | Cells were harvested at the indicated time points and washed three times with ice cold 5% mannitol. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR002052 |
| Treatment Summary: | Fully differentiated cultured brown adipocytes were treated with or without 1mM of d9-choline for 1, 6 or 24 hours. |
Sample Preparation:
| Sampleprep ID: | SP002046 |
| Sampleprep Summary: | Lipids were extracted using a methanol–chloroform–water extraction method. The methanol used contained 25 μM each of methionine sulfone and D-camphor-10-sulphonic acid and 10 μM each of 1,2-dimyristoyl(d54)-sn-glycero-3-phosphocholine and 1,2-dihexanoyl(d22)-sn-glycero-3-phosphocholine. |
Combined analysis:
| Analysis ID | AN003195 |
|---|---|
| Analysis type | MS |
| Chromatography type | Unspecified |
| Chromatography system | none |
| Column | none |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6530 QTOF |
| Ion Mode | POSITIVE |
| Units | fmol/cell |
Chromatography:
| Chromatography ID: | CH002363 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | Unspecified |
MS:
| MS ID: | MS002973 |
| Analysis ID: | AN003195 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Mass spectra were acquired at the rate of 1.5 cycles /sec over an mass range of m/z100–1,700. The reference masses of reference standards were 121.050873(Purine) and 922.009798(HP-0921) for positive ion mode, and 112.985587(TFA) and 1033.988109(HP-0921+TFA) for negative ion mode. Raw data from LC–TOFMS were processed using Master Hands ver. 2.17.0.8 software for the quantification of lipids. |
| Ion Mode: | POSITIVE |
