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MB Sample ID: SA183125
| Local Sample ID: | 2XH99 |
| Subject ID: | SU002015 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002015 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 2XH99 | SA183125 | FL022304 | Maligant SPNS | Pheotypes |
Collection:
| Collection ID: | CO002008 |
| Collection Summary: | A total of 1160 plasma samples was obtained from healthy volunteers (n=280), benign SPNs (n=157, center 1=42; center 2=23; center 3=45; center 4=13; center 5=34) and malignant SPNs (stage I, n=723, center 1=118; center 2=105; center 3=139; center 4=33; center 5=328) patients enrolled from 5 independent centers. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR002027 |
| Treatment Summary: | Without treatment |
Sample Preparation:
| Sampleprep ID: | SP002021 |
| Sampleprep Summary: | To undertake comprehensive lipidomic profiling, a sample preparation approach for serum based on liquid-liquid MTBE extraction was utilized to cover diverse classes of lipids. Untargeted lipidomic analysis was performed using a Dionex UltiMate 3000 UHPLC system (Santa Clara, CA, USA) linked online via electrospray ionization source (ESI) with a Q ExactiveTM Hybrid Quadrupole-OrbitrapTM Mass Spectrometer (Thermo Fisher Scientific, Inc., MA, USA). Comprehensive metabolomic analysis was performed on a TRACE 1310 gas chromatograph equipped with an AS 1310 autosampler connected to a TSQ 8000 triple quadrupole mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) as described previously 14. |
| Sampleprep Protocol ID: | 346 Lipidomics_LCMSProtocol.pdf |
Combined analysis:
| Analysis ID | AN003150 |
|---|---|
| Chromatography ID | CH002330 |
| MS ID | MS002930 |
| Analysis type | MS |
| Chromatography type | Unspecified |
| Chromatography system | Thermo Trace 1310/Dionex UltiMate 3000 UHPLC |
| Column | TG-5MS |
| MS Type | EI/ESI |
| MS instrument type | Triple quadrupole/Orbitrap |
| MS instrument name | Thermo TSQ 8000/Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | peak height |
Chromatography:
| Chromatography ID: | CH002330 |
| Instrument Name: | Thermo Trace 1310/Dionex UltiMate 3000 UHPLC |
| Column Name: | TG-5MS |
| Chromatography Type: | Unspecified |
MS:
| MS ID: | MS002930 |
| Analysis ID: | AN003150 |
| Instrument Name: | Thermo TSQ 8000/Thermo Q Exactive Orbitrap |
| Instrument Type: | Triple quadrupole/Orbitrap |
| MS Type: | EI/ESI |
| MS Comments: | The raw data files from LC-MS were converted into Analysis Base File (ABF) format by Abf Converter (http://www.reifycs.com/AbfConverter). Then, using the open-source program MS-Dial v.4.24 15, the automatic peak selecting, integration, retention time adjustment by the aforementioned database, and alignment were performed. The retention time index (RI) tolerance in GC-MS was 3,000, while the peak height threshold was 10,000. In LC-MS, the peak height threshold was 1,000,000 in positive ion mode and 500,000 in negative ion mode. The resultant output data table of high quality time-aligned investigated metabolites, together with their related RT, m/z, and peak height acquired for each sample, was statistically analyzed. All metabolite identifications were manually double-checked. |
| Ion Mode: | POSITIVE |
