Return to study ST001667 main page

MB Sample ID: SA152247

Local Sample ID:1--1
Subject ID:SU001744
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU001744
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
1--1SA152247FL017906ControlTreatment
1--1SA152247FL017906OvernightTimepoint

Collection:

Collection ID:CO001737
Collection Summary:Livers were removed from mice immediately following sacrifice and stored at -80 C. Before metabolite extraction, livers were homogenized into powder with mortar and pestle over liquid nitrogen.
Sample Type:Liver

Treatment:

Treatment ID:TR001757
Treatment Summary:Mice were injected via intraperitoneal (IP) route with either ricin or saline in 10 microliter per g body weight. Mice in all but the fasted group were given food and water ad libitum. Fasted mice were transferred to new cages with fresh bedding where they had food withheld while given water ad libitum. Mice were sacrificed at 2h (ricin), 8h (ricin and fasted), and overnight (16-22h, control, ricin, fasted).

Sample Preparation:

Sampleprep ID:SP001750
Sampleprep Summary:Microcentrifuge tubes with 30 mg of frozen liver tissue had 500 uL of ice-cold acetone and three Zirconia beads added to them. Tissues were lysed and homogenized, then sonicated and centrifuged. The supernatant was transferred to a new tube and 500 uL of ice-cold MeOH/H2O was added to the pellet. The pellet was sonicated and centrifuged again. The supernatant was combined with the acetone fraction and vacuum dried. Dried pellets were resuspended in acetonitrile/H2O with 1% Formic Acid, vortexed, sonicated, and centrifuged. 180 uL of eluate was used for LCMS analysis.

Combined analysis:

Analysis ID AN002719
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1290
Column Waters Acquity BEH HILIC (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode POSITIVE
Units Intensity

Chromatography:

Chromatography ID:CH002007
Instrument Name:Agilent 1290
Column Name:Waters Acquity BEH HILIC (150 x 2.1mm,1.7um)
Column Pressure:600 bar
Column Temperature:40
Flow Gradient:100% B, 55% B, 20% B, 100%B
Flow Rate:0.400 mL/min
Retention Time:25 min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:HILIC

MS:

MS ID:MS002516
Analysis ID:AN002719
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:mzML formatting acquired in ProteoWizard msconvert, Feature analysis performed in XCMS Online v3.7.1
Ion Mode:POSITIVE
  logo