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MB Sample ID: SA127495

Local Sample ID:PAG162_NonInf
Subject ID:SU001590
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:2-12 years
Weight Or Weight Range:11-30 Kilograms
Height Or Height Range:85 - 137 cm
Gender:Male and female
Human Ethnicity:Gouin (Burkina Faso, West Africa)
Human Inclusion Criteria:residency in the Nafona- and Bounouna rural area in Burkina Faso, age of 2 to 12 years, no P. falciparum infection at the time of enrollment (as evidenced by malaria rapid diagnostic test and confirmed later with microscopy and qPCR), no fever (axillary temperature ≥37.5°C) or history of fever within the preceding 48 hours
Human Exclusion Criteria:history of immunosuppressive diseases or chronic diseases, signs of concomitant febrile illness or active infection, use of anti-malarial treatment within the preceding two weeks, use of immunosuppressant or other immune-modifying drugs within the preceding six months and receiving blood transfusion within the preceding three months
Species Group:Mammals

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Subject:

Subject ID:SU001590
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:2-12 years
Weight Or Weight Range:11-30 Kilograms
Height Or Height Range:85 - 137 cm
Gender:Male and female
Human Ethnicity:Gouin (Burkina Faso, West Africa)
Human Inclusion Criteria:residency in the Nafona- and Bounouna rural area in Burkina Faso, age of 2 to 12 years, no P. falciparum infection at the time of enrollment (as evidenced by malaria rapid diagnostic test and confirmed later with microscopy and qPCR), no fever (axillary temperature ≥37.5°C) or history of fever within the preceding 48 hours
Human Exclusion Criteria:history of immunosuppressive diseases or chronic diseases, signs of concomitant febrile illness or active infection, use of anti-malarial treatment within the preceding two weeks, use of immunosuppressant or other immune-modifying drugs within the preceding six months and receiving blood transfusion within the preceding three months
Species Group:Mammals

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
PAG162_NonInfSA127495FL015706Non-infectedINFECTION

Collection:

Collection ID:CO001585
Collection Summary:Peripheral blood samples were collected in Serum Separator Tubes (SST). Serum samples were isolated on site in Burkina Faso by centrifuging blood samples in SST tubes for 30 minutes at 1500 RCF. Separated serum aliquots of 0.5 ml were transferred to cryovials and stored at -80°C.
Sample Type:Blood (serum)
Storage Conditions:-80℃
Collection Vials:Serum Separator Tubes (SST)
Storage Vials:cryovials

Treatment:

Treatment ID:TR001605
Treatment Summary:No treatment was done to any of the samples prior to profiling

Sample Preparation:

Sampleprep ID:SP001598
Sampleprep Summary:Samples were prepared using the automated MicroLab STAR® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.

Combined analysis:

Analysis ID AN002515 AN002516 AN002517
Analysis type MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity Waters Acquity
Column Waters Acquity BEH C18 (100 x 2mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um)
MS Type ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE UNSPECIFIED
Units raw area counts raw area counts raw area counts

Chromatography:

Chromatography ID:CH001839
Chromatography Summary:Low pH polar
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002333
Analysis ID:AN002515
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Proprietary analytical software for integration and peak picking
Ion Mode:POSITIVE
  
MS ID:MS002334
Analysis ID:AN002516
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Proprietary analytical software for integration and peak picking
Ion Mode:NEGATIVE
  
MS ID:MS002335
Analysis ID:AN002517
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Proprietary analytical software for integration and peak picking
Ion Mode:UNSPECIFIED
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