Return to study ST001414 main page

MB Sample ID: SA116094

Local Sample ID:71-2 1 9_10_1
Subject ID:SU001488
Subject Type:Bacteria
Subject Species:Pseudomonas aeruginosa
Taxonomy ID:287
Cell Biosource Or Supplier:Seattle Children's Hospital Cystic Fibrosis Isolate Core

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU001488
Subject Type:Bacteria
Subject Species:Pseudomonas aeruginosa
Taxonomy ID:287
Cell Biosource Or Supplier:Seattle Children's Hospital Cystic Fibrosis Isolate Core

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
71-2 1 9_10_1SA116094FL014436bacteriatreatment

Collection:

Collection ID:CO001483
Collection Summary:P. aeruginosa isolates were collected from 81 individuals with cystic fibrosis, and obtained from Seattle Children's Hospital Cystic Fibrosis Isolate Core. Bacterial isolates were stored as pure glycerol stocks at -70 degrees Celsius.
Sample Type:Bacterial cells

Treatment:

Treatment ID:TR001503
Treatment Summary:Liquid cultures of lysogeny broth (Lennox) were inoculated with P. aeruginosa from pure glycerol stocks and incubated aerobically at 37 degrees Celsius for 24 hours in triplicate. Cultures were centrifuged through a 0.2 ┬Ám filter to remove cells, and filtrates were transferred to GC headspace vials.

Sample Preparation:

Sampleprep ID:SP001496
Sampleprep Summary:Filtrate samples were heated to 50 degrees Celsius with agitation, and volatiles were sampled for 10 minutes using solid-phase microextraction (SPME) and analyzed using comprehensive gas chromatography coupled to time-of-flight mass spectrometry.
Processing Storage Conditions:Described in summary
Extraction Method:Solid-phase microextraction (SPME)

Combined analysis:

Analysis ID AN002364
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890B
Column Multidimensional configuration
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus 4D GCxGC TOF
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH001734
Chromatography Summary:Samples analyzed using comprehensive two-dimensional gas chromatography (GCxGC). See attached method file.
Methods Filename:tjdavi18_PR_CH_gcxgc_method.docx
Instrument Name:Agilent 7890B
Column Name:Multidimensional configuration
Chromatography Type:GC

MS:

MS ID:MS002206
Analysis ID:AN002364
Instrument Name:Leco Pegasus 4D GCxGC TOF
Instrument Type:GC x GC-TOF
MS Type:EI
MS Comments:Acquisition delay: 180 s Filament active time: 180 s to end of run Start mass/End mass: 35/400 Acquisition rate: 100 spectra/s Voltage offset: +50 V Electron energy: -70 eV Ion source temperature: 200 Celsius Acquisition masses: TIC Processing software: Leco ChromaTOF MS Library: NIST 2011
Ion Mode:POSITIVE
  logo