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MB Sample ID: SA116060
| Local Sample ID: | 74-2 2 9_13_1 |
| Subject ID: | SU001488 |
| Subject Type: | Bacteria |
| Subject Species: | Pseudomonas aeruginosa |
| Taxonomy ID: | 287 |
| Cell Biosource Or Supplier: | Seattle Children's Hospital Cystic Fibrosis Isolate Core |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001488 |
| Subject Type: | Bacteria |
| Subject Species: | Pseudomonas aeruginosa |
| Taxonomy ID: | 287 |
| Cell Biosource Or Supplier: | Seattle Children's Hospital Cystic Fibrosis Isolate Core |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 74-2 2 9_13_1 | SA116060 | FL014436 | bacteria | treatment |
Collection:
| Collection ID: | CO001483 |
| Collection Summary: | P. aeruginosa isolates were collected from 81 individuals with cystic fibrosis, and obtained from Seattle Children's Hospital Cystic Fibrosis Isolate Core. Bacterial isolates were stored as pure glycerol stocks at -70 degrees Celsius. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR001503 |
| Treatment Summary: | Liquid cultures of lysogeny broth (Lennox) were inoculated with P. aeruginosa from pure glycerol stocks and incubated aerobically at 37 degrees Celsius for 24 hours in triplicate. Cultures were centrifuged through a 0.2 µm filter to remove cells, and filtrates were transferred to GC headspace vials. |
Sample Preparation:
| Sampleprep ID: | SP001496 |
| Sampleprep Summary: | Filtrate samples were heated to 50 degrees Celsius with agitation, and volatiles were sampled for 10 minutes using solid-phase microextraction (SPME) and analyzed using comprehensive gas chromatography coupled to time-of-flight mass spectrometry. |
| Processing Storage Conditions: | Described in summary |
| Extraction Method: | Solid-phase microextraction (SPME) |
Combined analysis:
| Analysis ID | AN002364 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Multidimensional configuration |
| MS Type | EI |
| MS instrument type | GC x GC-TOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH001734 |
| Chromatography Summary: | Samples analyzed using comprehensive two-dimensional gas chromatography (GCxGC). See attached method file. |
| Methods Filename: | tjdavi18_PR_CH_gcxgc_method.docx |
| Instrument Name: | Agilent 7890B |
| Column Name: | Multidimensional configuration |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002206 |
| Analysis ID: | AN002364 |
| Instrument Name: | Leco Pegasus 4D GCxGC TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| MS Comments: | Acquisition delay: 180 s Filament active time: 180 s to end of run Start mass/End mass: 35/400 Acquisition rate: 100 spectra/s Voltage offset: +50 V Electron energy: -70 eV Ion source temperature: 200 Celsius Acquisition masses: TIC Processing software: Leco ChromaTOF MS Library: NIST 2011 |
| Ion Mode: | POSITIVE |
