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MB Sample ID: SA087763
| Local Sample ID: | LC44 |
| Subject ID: | SU001299 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001299 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| LC44 | SA087763 | FL012894 | LC | Disease |
Collection:
| Collection ID: | CO001293 |
| Collection Summary: | Morning fasting heparin anti-coagulated blood samples were drawn and centrifuged at 4°C, 3000 r / min for 10 min. The supernatant was aspirated, and frozen at -80°C. |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001314 |
| Treatment Summary: | No special treatment |
Sample Preparation:
| Sampleprep ID: | SP001307 |
| Sampleprep Summary: | The frozen heparin anti-coagulated plasma samples were thawed on ice and 100 μl of each sample was transferred to a 1.5ml sterile micro-centrifugen tube. After the addition of 300 μl of methanol (containing internal standard L-2-chlorophenylalanine 1 μg/ml), each sample was vortexed for 30 s, sonicated for 10 min in ice-water bath, and incubated for 1 h at -20°C to precipitate proteins. Then each sample was centrifuged at 12000 rpm for 15 min at 4°C. The resulting supernatants were transferred to LC-MS vials and stored at -80°C until the UPLC-QE-MS analysis. Each quality control sample was also prepared by mixing an equal aliquot of the supernatants from each sample. The treatment methods were the same as above. |
Combined analysis:
| Analysis ID | AN002048 | AN002049 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001488 |
| Chromatography Summary: | The mobile phase A was 0.1% formic acid in water for positive, and 5 mmol/L ammonium acetate in water for negative, and the mobile phase B was acetonitrile. The elution gradient was set as follows: 0 min, 1% B; 1 min, 1% B; 8 min, 99% B; 10 min, 99% B; 10.1 min, 1% B; 12 min, 1% B. |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Flow Gradient: | 0 min, 1% B; 1 min, 1% B; 8 min, 99% B; 10 min, 99% B; 10.1 min, 1% B; 12 min, 1% B. |
| Solvent A: | Pos mode:100% water; 0.1% formic acid, Neg mode:100% water; 5 mM ammonium acetate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001900 |
| Analysis ID: | AN002048 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | mass range(m/z):70-1000 Convert the mass spectrometry raw data to the mzML format. Then do retention time correction, peak identification, peak extraction, peak integration, peak alignment, etc., and finally compare the database for material identification. ProteoWizard,XCMS,OSI-SMMS,in-house MS/MS database |
| Ion Mode: | NEGATIVE |
| MS ID: | MS001901 |
| Analysis ID: | AN002049 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | mass range(m/z):70-1000 Convert the mass spectrometry raw data to the mzML format. Then do retention time correction, peak identification, peak extraction, peak integration, peak alignment, etc., and finally compare the database for material identification. ProteoWizard,XCMS,OSI-SMMS,in-house MS/MS database |
| Ion Mode: | POSITIVE |
