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MB Sample ID: SA080506
| Local Sample ID: | 125APE |
| Subject ID: | SU001229 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001229 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 125APE | SA080506 | FL012331 | A | Class |
Collection:
| Collection ID: | CO001223 |
| Collection Summary: | This study used EBC samples made available by the CF Biospecimen Registry, a part of the Emory+Children’s Center for CF and Airways Disease Research. EBC sample collection followed the guidelines approved by the Georgia Institute of Technology and the Emory University Institutional Review Boards (approval number IRB00000372). An R-Tube collector (Respiratory Research, Inc., Austin, TX, USA) was used to collect EBC samples, which were immediately frozen at -80 °C until processed. |
| Sample Type: | Breath |
Treatment:
| Treatment ID: | TR001244 |
| Treatment Summary: | Stable CF, APE and pre-APE patients |
Sample Preparation:
| Sampleprep ID: | SP001237 |
| Sampleprep Summary: | EBC samples were thawed and lyophilized at -40 °C and ~100 mTorr for 24 hours using a VirTis bench top freeze-dryer (SP Industries, Stone Ridge, NY, USA). Sample residues were reconstituted in water/methanol (90:10 v/v) with a concentration factor of 20, and analyzed by UPLC-MS. |
Combined analysis:
| Analysis ID | AN001925 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt G2 |
| Ion Mode | NEGATIVE |
| Units | Normalized peak area |
Chromatography:
| Chromatography ID: | CH001397 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001781 |
| Analysis ID: | AN001925 |
| Instrument Name: | Waters Synapt G2 |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | None |
| Ion Mode: | NEGATIVE |
