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MB Sample ID: SA043155

Local Sample ID:PooledUrine_MSMS_20
Subject ID:SU000811
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

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Subject:

Subject ID:SU000811
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
PooledUrine_MSMS_20SA043155FL008946MS/MSTreatment

Collection:

Collection ID:CO000805
Collection Summary:Urine was collected and frozen at -20 for storage. Prior to analysis, urine was thawed and centrifuged to remove particulates. Synthetic NACIP-gl material was stored in water at 4 and diluted in water prior to analysis.
Sample Type:Urine

Treatment:

Treatment ID:TR000825
Treatment Summary:Urine, urine spiked with synthetic standard, and synthetic standard alone were analyzed by LC-MS and LC-MS/MS

Sample Preparation:

Sampleprep ID:SP000818
Sampleprep Summary:Urine was thawed on ice and centrifuged to remove particulates prior to analysis. N1-acetylisoputreanine-γ-lactam was synthesized as previously described. Acetylation was performed with N-(3-Aminopropyl) pyrrolidin-2-one (0.75 M) and acetyl chloride (0.75 M) in methanol and 0.5 M PBS (1:1) adjusted to pH 7.0 with sodium hydroxide. The reaction products were extracted with dichloromethane, dried, suspended in 2 ml LC-MS grade water and stored at 4˚C. Prior to LC-MS analyses, the synthetic compound was diluted 1:100 in water or human urine.

Combined analysis:

Analysis ID AN001245
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1200
Column Atlantis T3 reverse-phase C18 (150 x 2.1mm,3.5um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent
Ion Mode POSITIVE
Units counts*min

Chromatography:

Chromatography ID:CH000869
Instrument Name:Agilent 1200
Column Name:Atlantis T3 reverse-phase C18 (150 x 2.1mm,3.5um)
Column Temperature:30
Flow Gradient:100% Solvent A to 90% Solvent B
Flow Rate:0.25 ml/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS001138
Analysis ID:AN001245
Instrument Name:Agilent
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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