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MB Sample ID: SA043152
| Local Sample ID: | Urine-ACISOGA_012417 |
| Subject ID: | SU000811 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000811 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Urine-ACISOGA_012417 | SA043152 | FL008945 | MS | Treatment |
Collection:
| Collection ID: | CO000805 |
| Collection Summary: | Urine was collected and frozen at -20 for storage. Prior to analysis, urine was thawed and centrifuged to remove particulates. Synthetic NACIP-gl material was stored in water at 4 and diluted in water prior to analysis. |
| Sample Type: | Urine |
Treatment:
| Treatment ID: | TR000825 |
| Treatment Summary: | Urine, urine spiked with synthetic standard, and synthetic standard alone were analyzed by LC-MS and LC-MS/MS |
Sample Preparation:
| Sampleprep ID: | SP000818 |
| Sampleprep Summary: | Urine was thawed on ice and centrifuged to remove particulates prior to analysis. N1-acetylisoputreanine-γ-lactam was synthesized as previously described. Acetylation was performed with N-(3-Aminopropyl) pyrrolidin-2-one (0.75 M) and acetyl chloride (0.75 M) in methanol and 0.5 M PBS (1:1) adjusted to pH 7.0 with sodium hydroxide. The reaction products were extracted with dichloromethane, dried, suspended in 2 ml LC-MS grade water and stored at 4˚C. Prior to LC-MS analyses, the synthetic compound was diluted 1:100 in water or human urine. |
Combined analysis:
| Analysis ID | AN001245 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1200 |
| Column | Atlantis T3 reverse-phase C18 (150 x 2.1mm,3.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent |
| Ion Mode | POSITIVE |
| Units | counts*min |
Chromatography:
| Chromatography ID: | CH000869 |
| Instrument Name: | Agilent 1200 |
| Column Name: | Atlantis T3 reverse-phase C18 (150 x 2.1mm,3.5um) |
| Column Temperature: | 30 |
| Flow Gradient: | 100% Solvent A to 90% Solvent B |
| Flow Rate: | 0.25 ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001138 |
| Analysis ID: | AN001245 |
| Instrument Name: | Agilent |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
