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MB Sample ID: SA035958

Local Sample ID:ms6103-20
Subject ID:SU000660
Subject Type:Rat
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammal

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Subject:

Subject ID:SU000660
Subject Type:Rat
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
ms6103-20SA035958FL007544YCR1 kidney cortexsample source
ms6103-20SA035958FL007544Transgenic Negativegroup

Collection:

Collection ID:CO000654
Collection Summary:The objective of the study is to identify Fumarase in hypertension. Samples are from Transgenic fumerase rats and WT littermates, 13 weeks of age
Sample Type:Kidney

Treatment:

Treatment ID:TR000674
Treatment Summary:We will analyze kidney tissue extract and urine samples from SS rats and the newly generated SS.Fh1+ transgenic rats. The SS.Fh1+ rat was generated using a Sleeping Beauty transposon-mediated transgenic technique. Overexpression of fumarase has been confirmed in SS.Fh1+ rats. Preliminary study indicated that the development of salt-induced hypertension was altered in SS.Fh1+ rats compared to wild-type littermate SS rats. We will analyze kidney tissue extract and urine samples collected from rats maintained on a 0.4% NaCl diet or switched to a 4% NaCl diet for 7 days. The dietary protocol has been used in numerous studies to examine mechanisms underlying salt sensitivity including early responses (7 days) to salt in the SS model. In total, 64 rat samples (two rat strains, two salt levels, and 8 rats per condition) will be analyzed.

Sample Preparation:

Sampleprep ID:SP000667
Sampleprep Summary:Rat urine and kidney tissue extract (outer medulla and cortex) TCA concentrations. Urine TCA concentrations in uM and tissue in nmol/vial

Combined analysis:

Analysis ID AN000969
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890B
Column Agilent HP5-MS (30m × 0.25mm, 0.25 um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975C
Ion Mode POSITIVE
Units urine uM and Tissue nnol/vial

Chromatography:

Chromatography ID:CH000694
Instrument Name:Agilent 7890B
Column Name:Agilent HP5-MS (30m × 0.25mm, 0.25 um)
Chromatography Type:GC

MS:

MS ID:MS000864
Analysis ID:AN000969
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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