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MB Sample ID: SA035327

Local Sample ID:ms5841-2
Subject ID:SU000653
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

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Subject:

Subject ID:SU000653
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
ms5841-2SA035327FL007523LSodium level
ms5841-2SA035327FL007523A(salt sensitive)grouping

Collection:

Collection ID:CO000647
Collection Summary:The urine samples we have were 24-hour collections occurring in the fourth week on either the low- or the high-sodium intake level. In total, 120 human samples (60 subjects, a low-sodium sample and a high-sodium sample for each subject) will be analyzed.
Sample Type:Urine

Treatment:

Treatment ID:TR000667
Treatment Summary:We will analyze urine specimens collected during the Dietary Approaches to Stop Hypertension – Sodium Study (DASH-Sodium or DASH2) clinical trial. More than 400 participants in the study were divided into two groups. One followed a common Western diet, while the other group followed the “healthier” DASH diet. Then each group had three 30 day periods, in random orders, that corresponded to high, intermediate, or low sodium intakes while eating their designated diet. The participants had all of their meals prepared and provided for them during the entire study. It was found that reducing sodium intake significantly reduced blood pressure especially when on the common Western diet. We have obtained urine samples and phenotypic data for a subset of the participants of the DASH2 trial form the NHLBI biospecimen and data repository BioLINCC. We selected two sub-groups of participants on the Western diet. Group A (n=31) exhibited large (>12 mmHg) differences in systolic or diastolic pressure between low- and high-sodium intakes (“salt-sensitive” group). Group B (n=29) showed similar blood pressure (differences <5 mmHg for both systolic and diastolic pressures) between low- and high-sodium intakes (“saltinsensitive” group).

Sample Preparation:

Sampleprep ID:SP000660
Sampleprep Summary:2 drops of 6 N HCl was added in every 8 ml urine samples when the 24 hour urine was collected. All urine samples were stored in -80℃ freezer.

Combined analysis:

Analysis ID AN000962
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantum Ultra
Ion Mode POSITIVE
Units uM

Chromatography:

Chromatography ID:CH000687
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000857
Analysis ID:AN000962
Instrument Name:Thermo Quantum Ultra
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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