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MB Sample ID: SA034337
| Local Sample ID: | HR06_1 |
| Subject ID: | SU000641 |
| Subject Type: | Human Follicular Fluid |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | female |
| Human Exclusion Criteria: | cancer, endometriosis, premature ovarian failure |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000641 |
| Subject Type: | Human Follicular Fluid |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | female |
| Human Exclusion Criteria: | cancer, endometriosis, premature ovarian failure |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| HR06_1 | SA034337 | FL007479 | Hyper Responder | Condition |
Collection:
| Collection ID: | CO000635 |
| Collection Summary: | The patients were initially undergoing to controlled ovarian stimulation, from which follicular fluid samples were collected. |
| Sample Type: | Follicular fluid |
Treatment:
| Treatment ID: | TR000655 |
| Treatment Summary: | Follicular fluid samples were centrifuged at 800 × g for 10 minutes to separate the fluid from follicular cells and the samples were maintained frozen in -20 °C until the metabolites extraction. |
Sample Preparation:
| Sampleprep ID: | SP000648 |
| Sampleprep Summary: | 50 µL of follicular fluid samples were submitted to Bligh & Dyer protocol. |
| Extraction Method: | BLIGH & DYER |
| Sample Resuspension: | 5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v) |
Combined analysis:
| Analysis ID | AN000948 |
|---|---|
| Chromatography ID | CH000675 |
| MS ID | MS000844 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Ultima Quadrupole-Time of Flight - Waters Corporation |
| Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Ultima QTOF |
| Ion Mode | POSITIVE |
| Units | mass intensity |
Chromatography:
| Chromatography ID: | CH000675 |
| Chromatography Summary: | Liquid Chromatography was performed by using a chromatographer Acquity with a reverse phase column (Acquity UPCL HSS 1,8 µm, 2.1x100 mm - Waters Corporation, MA, USA), at 35 °C. The solvents for the elution were: A- H2O with formic acid 0.1% and B- ACN with formic acid 0.1%. The initial gradient was composed of 80% of A and 20% of B, during 1 minute. Next, the gradient was linearly changed for 50% of A and 50% of B, during the next minute. In the sequence, the gradient was changed for 35% of A and 65% of B during 2 minutes. The gradient was then inverted, considering 20% of A and 80% of B, during 3 minutes. For the next 10 minutes, the gradient was 3% of A and 97% of B. The initial condition was reestablished for the column washing during 1 minute. The total run time was 17 minutes and the solvents flux was 0.5 mL/min and the total amount of sample used per run was 5 µL. |
| Instrument Name: | Ultima Quadrupole-Time of Flight - Waters Corporation |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 35 °C |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000844 |
| Analysis ID: | AN000948 |
| Instrument Name: | Waters Ultima QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
