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MB Sample ID: SA034235

Local Sample ID:C15_1
Subject ID:SU000641
Subject Type:Human Follicular Fluid
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:female
Human Exclusion Criteria:cancer, endometriosis, premature ovarian failure
Species Group:Human

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Subject:

Subject ID:SU000641
Subject Type:Human Follicular Fluid
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:female
Human Exclusion Criteria:cancer, endometriosis, premature ovarian failure
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
C15_1SA034235FL007478ControlCondition

Collection:

Collection ID:CO000635
Collection Summary:The patients were initially undergoing to controlled ovarian stimulation, from which follicular fluid samples were collected.
Sample Type:Follicular fluid

Treatment:

Treatment ID:TR000655
Treatment Summary:Follicular fluid samples were centrifuged at 800 × g for 10 minutes to separate the fluid from follicular cells and the samples were maintained frozen in -20 °C until the metabolites extraction.

Sample Preparation:

Sampleprep ID:SP000648
Sampleprep Summary:50 µL of follicular fluid samples were submitted to Bligh & Dyer protocol.
Extraction Method:BLIGH & DYER
Sample Resuspension:5 µL of ressuspended sample in 100 µL of ACN:H20 (1:1 - v:v)

Combined analysis:

Analysis ID AN000948
Analysis type MS
Chromatography type Reversed phase
Chromatography system Ultima Quadrupole-Time of Flight - Waters Corporation
Column Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Waters Ultima QTOF
Ion Mode POSITIVE
Units mass intensity

Chromatography:

Chromatography ID:CH000675
Chromatography Summary:Liquid Chromatography was performed by using a chromatographer Acquity with a reverse phase column (Acquity UPCL HSS 1,8 µm, 2.1x100 mm - Waters Corporation, MA, USA), at 35 °C. The solvents for the elution were: A- H2O with formic acid 0.1% and B- ACN with formic acid 0.1%. The initial gradient was composed of 80% of A and 20% of B, during 1 minute. Next, the gradient was linearly changed for 50% of A and 50% of B, during the next minute. In the sequence, the gradient was changed for 35% of A and 65% of B during 2 minutes. The gradient was then inverted, considering 20% of A and 80% of B, during 3 minutes. For the next 10 minutes, the gradient was 3% of A and 97% of B. The initial condition was reestablished for the column washing during 1 minute. The total run time was 17 minutes and the solvents flux was 0.5 mL/min and the total amount of sample used per run was 5 µL.
Instrument Name:Ultima Quadrupole-Time of Flight - Waters Corporation
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:35 °C
Flow Rate:0.5 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS000844
Analysis ID:AN000948
Instrument Name:Waters Ultima QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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