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MB Sample ID: SA012055

Local Sample ID:Rv3244_C_15
Subject ID:SU000285
Subject Type:Bacterial cells
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332
Genotype Strain:Mycobacterium tuberculosis H37Rv, Rv3244
Species Group:Microorganism

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Subject:

Subject ID:SU000285
Subject Type:Bacterial cells
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332
Genotype Strain:Mycobacterium tuberculosis H37Rv, Rv3244
Species Group:Microorganism

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Rv3244_C_15SA012055FL00521215timepoint (min)

Collection:

Collection ID:CO000279
Collection Summary:protein expression
Collection Protocol ID:Rv3244 from M. tuberculosis H37Rv was expressed in E. coli cells and purified...
Sample Type:Bacterial cells
Collection Vials:polypropylene microfuge tubes
Storage Vials:Agilent polypropylene conical bottom vials

Treatment:

Treatment ID:TR000299

Sample Preparation:

Sampleprep ID:SP000293
Sampleprep Summary:10 uM enzyme incubated at 25C in M. bovis whole-cell extract
Processing Method:extract was lyophilized and resuspended in 20 mM Tris-HCL pH 8.0
Processing Storage Conditions:cell pellet frozen at -80C
Extraction Method:70% acetonitrile
Extract Cleanup:centrifugation followed by aspiration of supernatant and lyophilization
Extract Storage:frozen at -80C

Combined analysis:

Analysis ID AN000423 AN000424
Analysis type MS MS
Chromatography type Normal phase Normal phase
Chromatography system Agilent 1290 Agilent 1290
Column Microsolv Diamond hydride (150mm,4um) Microsolv Diamond hydride (150mm,4um)
MS Type ESI ESI
MS instrument type TOF TOF
MS instrument name Agilent 6220 TOF Agilent 6220 TOF
Ion Mode POSITIVE NEGATIVE
Units integrated peak counts integrated peak counts

Chromatography:

Chromatography ID:CH000297
Chromatography Summary:Aqueous normal phase diamond hydride
Methods Filename:ANPpos
ANP_neg
Instrument Name:Agilent 1290
Column Name:Microsolv Diamond hydride (150mm,4um)
Flow Rate:0.4 ml/min
Solvent A:100% water; 0.2% formic acid
Solvent B:100% acetonitrile; 0.2% formic acid
Analytical Time:24 minutes
Washing Buffer:50% methanol
Chromatography Type:Normal phase

MS:

MS ID:MS000364
Analysis ID:AN000423
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Voltage:3500
Dry Gas Flow:10 l/min
Dry Gas Temp:250
Fragment Voltage:135V
Mass Accuracy:5 ppm
Nebulizer:35
Octpole Voltage:400
Scan Range Moverz:50-1200
Scanning Cycle:1.4
Skimmer Voltage:65
  
MS ID:MS000365
Analysis ID:AN000424
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Voltage:3500
Dry Gas Flow:10 l/min
Dry Gas Temp:250
Fragment Voltage:135V
Mass Accuracy:5 ppm
Nebulizer:35
Octpole Voltage:400
Scan Range Moverz:50-1200
Scanning Cycle:1.4
Skimmer Voltage:65
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