Return to study ST000262 main page

MB Sample ID: SA012025

Local Sample ID:1mMGTP1713_C_0
Subject ID:SU000282
Subject Type:Bacterial cells
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332
Genotype Strain:Mycobacterium tuberculosis H37Rv, taxid:
Species Group:Microorganism

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU000282
Subject Type:Bacterial cells
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332
Genotype Strain:Mycobacterium tuberculosis H37Rv, taxid:
Species Group:Microorganism

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
1mMGTP1713_C_0SA012025FL0029580timepoint (min)
1mMGTP1713_C_0SA012025FL0029581mM GTPco-factor added to lysate

Collection:

Collection ID:CO000276
Collection Summary:protein expression
Collection Protocol ID:Rv1713 from M. tuberculosis H37Rv was expressed in E. coli cells and purified...
Sample Type:Bacterial cells
Collection Vials:polypropylene microfuge tubes
Storage Vials:Agilent polypropylene conical bottom vials

Treatment:

Treatment ID:TR000296

Sample Preparation:

Sampleprep ID:SP000290
Sampleprep Summary:10 uM enzyme incubated at 25C in M. bovis whole-cell extract
Processing Method:extract was lyophilized and resuspended in 20 mM Tris-HCL pH 8.0
Processing Storage Conditions:cell pellet frozen at -80C
Extraction Method:70% acetonitrile
Extract Cleanup:centrifugation followed by aspiration of supernatant and lyophilization
Extract Storage:frozen at -80C

Combined analysis:

Analysis ID AN000417 AN000418
Analysis type MS MS
Chromatography type Normal phase Normal phase
Chromatography system Agilent 1290 Agilent 1290
Column Microsolv Diamond hydride (150mm,4um) Microsolv Diamond hydride (150mm,4um)
MS Type ESI ESI
MS instrument type TOF TOF
MS instrument name Agilent 6220 TOF Agilent 6220 TOF
Ion Mode POSITIVE NEGATIVE
Units integrated peak counts integrated peak counts

Chromatography:

Chromatography ID:CH000294
Chromatography Summary:Aqueous normal phase diamond hydride
Methods Filename:ANPpos
ANP_neg
Instrument Name:Agilent 1290
Column Name:Microsolv Diamond hydride (150mm,4um)
Flow Rate:0.4 ml/min
Solvent A:100% water; 0.2% formic acid
Solvent B:100% acetonitrile; 0.2% formic acid
Analytical Time:24 minutes
Washing Buffer:50% methanol
Chromatography Type:Normal phase

MS:

MS ID:MS000358
Analysis ID:AN000417
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Voltage:3500
Dry Gas Flow:10 l/min
Dry Gas Temp:250
Fragment Voltage:135V
Mass Accuracy:5 ppm
Nebulizer:35
Octpole Voltage:400
Scan Range Moverz:50-1200
Scanning Cycle:1.4
Skimmer Voltage:65
  
MS ID:MS000359
Analysis ID:AN000418
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Voltage:3500
Dry Gas Flow:10 l/min
Dry Gas Temp:250
Fragment Voltage:135V
Mass Accuracy:5 ppm
Nebulizer:35
Octpole Voltage:400
Scan Range Moverz:50-1200
Scanning Cycle:1.4
Skimmer Voltage:65
  logo