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MB Sample ID: SA010908

Local Sample ID:1130_B_20
Subject ID:SU000253
Subject Type:Bacterial cells
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332
Species Group:Microorganism

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU000253
Subject Type:Bacterial cells
Subject Species:Mycobacterium tuberculosis H37Rv
Taxonomy ID:83332
Species Group:Microorganism

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
1130_B_20SA010908FL00265420timepoint (min)

Collection:

Collection ID:CO000243
Collection Summary:protein expression
Collection Protocol ID:Rv1130 from M. tuberculosis H37Rv was expressed in E. coli cells and purified...
Sample Type:Bacterial cells
Collection Vials:polypropylene microfuge tubes
Storage Vials:Agilent polypropylene conical bottom vials

Treatment:

Treatment ID:TR000263

Sample Preparation:

Sampleprep ID:SP000257
Sampleprep Summary:10 uM enzyme incubated at 37C in M. bovis whole-cell extract
Processing Method:extract was lyophilized and resuspended in 20 mM Tris-HCL pH 8.0
Processing Storage Conditions:cell pellet frozen at -80C
Extraction Method:70% acetonitrile
Extract Cleanup:centrifugation followed by aspiration of supernatant and lyophilization
Extract Storage:frozen at -80C

Combined analysis:

Analysis ID AN000351
Analysis type MS
Chromatography type Normal phase
Chromatography system Agilent 1290
Column Microsolv Diamond hydride (150mm,4um)
MS Type ESI
MS instrument type TOF
MS instrument name Agilent 6220 TOF
Ion Mode POSITIVE
Units Peak intensity

Chromatography:

Chromatography ID:CH000261
Chromatography Summary:Aqueous normal phase diamond hydride
Methods Filename:ANPpos
ANP_neg
Instrument Name:Agilent 1290
Column Name:Microsolv Diamond hydride (150mm,4um)
Flow Rate:0.4 ml/min
Solvent A:100% water; 0.2% formic acid
Solvent B:100% acetonitrile; 0.2% formic acid
Analytical Time:24 minutes
Washing Buffer:50% methanol
Chromatography Type:Normal phase

MS:

MS ID:MS000297
Analysis ID:AN000351
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:3500
Collision Gas:10 l/min
Dry Gas Flow:250
Dry Gas Temp:135V
Ionization Potential:5 ppm
Matrix:35
Nebulizer:400
Sample Dripping:50-1200
Scanning:1.4
Scanning Range:65
  
MS ID:MS000298
Analysis ID:AN000352
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Temperature:3500
Collision Gas:10 l/min
Dry Gas Flow:250
Dry Gas Temp:135V
Ionization Potential:5 ppm
Matrix:35
Nebulizer:400
Sample Dripping:50-1200
Scanning:1.4
Scanning Range:65
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