Summary of project PR001509

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Project ID: PR001509
Project DOI:doi: 10.21228/M8N71K
Project Title:Biomolecular condensates create phospholipid-enriched microenvironments
Project Type:Metabolomics of in vitro condensates
Project Summary:Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1.
Institute:Cornell University
Department:Department of Pharmacology
Laboratory:Dr. Samie Jaffrey
Last Name:Dumelie
First Name:Jason
Address:1300 York Ave, LC-524, New York City, NY
Email:jdumes98@gmail.com
Phone:6465690174
Funding Source:This work was supported by the National Institutes of Health grants R35NS111631 and R01CA186702 (S.R.J.); R01AR076029, R21ES032347 and R21NS118633 (Q.C.); and NIH P01 HD067244 and support from the Starr Cancer Consortium I13-0037 (S.S.G.).
Publications:Under revision
Contributors:Jason G. Dumelie, Qiuying Chen, Dawson Miller, Nabeel Attarwala, Steven S. Gross and Samie R. Jaffrey1

Summary of all studies in project PR001509

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
Release
Date
VersionSamplesDownload
(* : Contains raw data)
ST002349 Biomolecular condensates create phospholipid-enriched microenvironments Mus musculus Cornell University MS 2022-11-28 1 39 Uploaded data (2.7G)*
ST002352 Biomolecular condensates create phospholipid-enriched microenvironments (Part 2) Mus musculus Cornell University MS 2022-12-06 1 18 Uploaded data (107.9M)*
ST002353 Biomolecular condensates create phospholipid-enriched microenvironments (Part 3) Cornell University MS 2022-12-06 1 27 Uploaded data (231M)*
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