Summary of project PR001424

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001424. The data can be accessed directly via it's Project DOI: 10.21228/M8NM68 This work is supported by NIH grant, U2C- DK119886.


Project ID: PR001424
Project DOI:doi: 10.21228/M8NM68
Project Title:U2OS Etoposide-PRDX1 metabolomics
Project Type:MS quantitative analysis
Project Summary:A metabolic map of the DNA damage response identifies PRDX1 in nuclear ROS scavenging and aspartate synthesis. While cellular metabolism impacts the DNA damage response, a systematic understanding of the metabolic requirements that are crucial for DNA damage repair has yet to be achieved. Here, we investigate the metabolic enzymes and processes that are essential when cells are exposed to DNA damage. By integrating functional genomics with chromatin proteomics and metabolomics, we provide a detailed description of the interplay between cellular metabolism and the DNA damage response. Subsequent analysis identified Peroxiredoxin 1, PRDX1, as fundamental for DNA damage repair. During the DNA damage response, PRDX1 translocates to the nucleus where it is required to reduce DNA damage-induced nuclear reactive oxygen species levels. Moreover, PRDX1 controls aspartate availability, which is required for the DNA damage repair-induced upregulation of de novo nucleotide synthesis. Loss of PRDX1 leads to an impairment in the clearance of γΗ2ΑΧ nuclear foci, accumulation of replicative stress and cell proliferation defects, thus revealing a crucial role for PRDX1 as a DNA damage surveillance factor.
Laboratory:Sdelci lab
Last Name:Kourtis
First Name:Savvas
Address:Carrer del Dr. Aiguader, 88, 08003 Barcelona

Summary of all studies in project PR001424

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST002234 A metabolic map of the DNA damage response identifies PRDX1 in nuclear ROS scavenging and aspartate synthesis Homo sapiens CRG MS 2023-04-03 1 30 Uploaded data (742.1M)*