Summary of project PR001383

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001383. The data can be accessed directly via it's Project DOI: 10.21228/M8Z993 This work is supported by NIH grant, U2C- DK119886.


Project ID: PR001383
Project DOI:doi: 10.21228/M8Z993
Project Title:Identifying a tryptophan derivative in hydrogen peroxide-treated cell culture medium
Project Summary:Reactive oxygen species (ROS) are by-products of metabolism of oxygen and they play an important role in normal homeostasis and cell signaling, as well as in the initiation of diseases including cancer when their production is upregulated. Thus, it is imperative to understand the cellular and molecular basis by which ROS impact on various biological and pathological processes. Here, we identified 2-oxindole, a tryptophan derivative, was a major catabolic product in hydrogen peroxide-treated cell culture medium. We used 2-oxindole to study its role in regulating AhR signaling and tryptophan metabolic pathways. We found that 2-oxindole significantly increased the activity of AhR, leading to enhanced expression of its downstream targets including cytochrome P450 genes.
Institute:NYU Langone Health
Department:Department of Biochemistry and Molecular Pharmacology
Last Name:Choi
First Name:Byeong Hyeok
Address:341 East 25 Street New York, NY 10010
Funding Source:NCI/US Public Service Award
Contributors:Metabolomics Core Resource Laboratory

Summary of all studies in project PR001383

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST002174 Identifying a tryptophan derivative in hydrogen peroxide-treated cell culture medium NYU Langone Health MS 2022-06-01 1 12 Uploaded data (303.6M)*