Summary of project PR000764

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000764. The data can be accessed directly via it's Project DOI: 10.21228/M8XH5B This work is supported by NIH grant, U2C- DK119886.


Project ID: PR000764
Project DOI:doi: 10.21228/M8XH5B
Project Title:Microbial depletion and ozone exposure
Project Summary:Ozone is an asthma trigger. In mice, the gut microbiome contributes to ozone-induced airway hyperresponsiveness, a defining feature of asthma. The purpose of this study was to identify metabolites that could be mediating this role. Gut bacterial enzymes modify ingested substances producing metabolites that enter the blood and circulate to host tissues where they may exert a variety of effects. Therefore, we performed global metabolomic profiling on serum of mice after acute ozone exposure. To identify the role of the microbiome in mediating ozone-induced metabolomic changes, mice were treated for two weeks with a cocktail of antibiotics in the drinking water or with control water and then exposed to air or ozone (2 ppm for 3 hours). Twenty four hours later, blood was harvested and serum analyzed via liquid-chromatography or gas-chromatography coupled to mass spectrometry. We observed marked effects of both ozone exposure and antibiotics on the serum metabolome. Known bacterially-derived metabolites were reduced in antibiotic-treated mice. Importantly, our data also indicated that ozone-induced changes in serum lipids, including long chain fatty acids and bile acids, as well as ozone-induced changes in polyamines were different in control and antibiotic-treated mice. Each of these metabolites has the capacity to alter airway responsiveness and may account for the role of the microbiome in pulmonary responses to ozone.
Institute:Harvard School of Public Health
Department:Molecular and Integrative Physiological Sciences
Last Name:Shore
First Name:Stephanie
Address:677 Huntington Ave

Summary of all studies in project PR000764

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST001143 Microbial depletion and ozone exposure - Lung tissue (part I) Mus musculus Harvard School of Public Health MS 2019-05-15 1 32 Uploaded data (7.1M)*
ST001144 Microbial depletion and ozone exposure - serum (part II) Mus musculus Harvard School of Public Health MS 2019-05-15 1 32 Uploaded data (7.1M)*