Summary of project PR000327

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000327. The data can be accessed directly via it's Project DOI: 10.21228/M8GS39 This work is supported by NIH grant, U2C- DK119886.


Project ID: PR000327
Project DOI:doi: 10.21228/M8GS39
Project Title:The role of CFTR in the regulation of intrinsic defense mechanisms of exocrine secretions
Project Summary:This study was designed as a pilot to determine if the concentrations of selected ions in salivary gland secretions were influenced by the absence of CFTR on the apical surfaces of glandular cells and ductal epithelia. To this end, five cystic fibrosis children homozygous for Phe508 were to be recruited with their heterozygous non-CF mothers. Unstimulated glandular secretions to include parotid and submandibular/sublingual saliva samples as well as whole, mixed saliva collected with masticatory stimulation were to be collected from the child and the mother and analyzed for nitrate (NO3), nitrite (NO2), bicarbonate (HCO3), thiocyanate (SCN), reduced and oxidized glutathione (GSH and GSSG) and buffering capacity (pH before and after addition of HCl to 3.75mN). For adaptation and development of the assays to a microtiter format, saliva samples were collected from non-CF control subjects initially to optimize the assays recognizing that the volumes for analyses would be small. The values obtained with these non-CF control samples were also compared to that of the homozygous and heterozygous CF subjects. As residual volume permits, these samples will be further analyzed for metabolic constituent profiles.
Institute:University of North Carolina at Chapel Hill
Department:School of Dentistry
Last Name:Arnold
First Name:Roland
Address:385 S Columbia Street, Chapel Hill, NC 27599

Summary of all studies in project PR000327

Study IDStudy TitleSpeciesInstituteAnalysis
(* : Contains Untargted data)
(* : Contains raw data)
ST000418 The role of CFTR in the regulation of intrinsic defense mechanisms of exocrine secretions Homo sapiens University of North Carolina MS 2018-06-05 1 22 Uploaded data (162.6M)*